Methods in Enzymology Vol.64 Enzyme Kinetics and Mechanism, Part B: Isotopic Probes and Complex

This chapter discusses that isotope exchange methods constitute an entire domain of enzyme kinetics that until 20 years ago was largely concerned with net velocity measurements. The availability of isotopes and appropriate sensing devices has encouraged the development of new approaches to understand enzyme catalysis and regulation. It deals with representative applications of isotopic exchange at or near equilibrium. It also explains the exchange processes away from equilibrium, a condition of obvious importance, when one studies irreversible or essentially the irreversible processes. Again, valuable information, frequently obtained in such cases, includes the order of substrate binding and product release, the participation of covalent enzyme-substrate intermediates, and the nature of the irreversible step. The chapter discusses the application of isotopic exchange methods to understand the biological processes. Such diverse topics, as energy transduction, allosteric regulation, enzyme cooperativity, metabolic transport, and the behavior of supramolecular processes, have all been enriched by the clever application of isotope exchange measurements. It reviews that the power and scope of the technique is still undergoing rapid expansion in theory and experiment.

• Series: Methods in Enzymology (Book 64)
• Hardcover: 418 pages
• Publisher: Academic Press; 1 edition (April 11, 1980)
• Language: English
• ISBN-10: 0121819647
• ISBN-13: 978-0121819644
• Product Dimensions: 6 x 1 x 9 inches

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