The principles underlying the various assays of acetyl-CoA carboxylase have been described in previous articles in this series. H Most conveniently, the enzyme activity is determined by the 14CO2-fixation assay or by the spectrophotometric assay in combination with the pyruvate kinase and lactate dehydrogenase reactions. The 14CO2-fixation assay can be used for enzyme preparations from all steps, whereas the spec- trophotometric assay is applicable to preparations from the DEAE- cellulose chromatography step and subsequent steps Series: Methods in Enzymology (Book 71) Hardcover: 896 pages Publisher: Academic Press Language: English ISBN-10: 012181971X ISBN-13: 978-0121819712 Product Dimensions: 6 x 1.8 x 9 inches Link Download http://nitroflare.com/view/A38D9EBF0BA6F4Dhttps://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
