[IMG] Radiolabeling of the interferons (IFNs) is a prerequisite for the studies of their binding and internalization. With the purification and...
[IMG] This chapter describes the methods used for isolating and characterizing the noncellulosic polysaccharides of the primary walls of...
[IMG] The development of sedimentation equilibrium in the air-driven preparative ultracentrifuge has provided an independent, nonempirical method...
[IMG] This chapter provides an overview of immunoglobulin G (IgG). IgG molecules consist of identical pairs of heavy (H) and light (L) chains (MW...
[IMG] This chapter discusses a book “Methods in Enzymology” that presents as comprehensively as possible current techniques used in biochemistry,...
[IMG] In the 1930s, when biochemists began to realize that all chemical reactions in living cells are catalyzed by enzymes, the secret of life was...
[IMG] γ-Aminobutyric acid (GABA) is one of the major neurotransmitters in the vertebrate central nervous system. L-Glutamate decarboxylase (GAD),...
[IMG] Ralph Green is a descendant of several Confederate veterans. Born in Dallas, Texas, he graduated from SMU after his service in the U.S. Army...
[IMG] There is a wide variety of methods available for the identification and quantitation of sterols. This chapter summarizes the usefulness of...
[IMG] Fifteen years after its introduction, affinity chromatography, la,2 a method of purification based on biological recognition, has become a...
[IMG] A survey of the current biological literature will convince even the most skeptical individual of the substantive and pervasive advances...
[IMG] This chapter discusses the historical perspective of calmodulin purification and the strategies behind approaching the purification of...
[IMG] Exciting new developments in recombinant DNA research allow the isolation and amplification of specific genes or DNA segments from almost...
[IMG] This chapter discusses the assays of the protein kinase. The radioisotopic method for cAMP-dependent protein kinase can be used with tissue...
[IMG] FROM THE PREFACE: Volumes 96 to 98, Parts J, K, and L of the Biomembranes series, focus on methodology to study membrane biogenesis,...
[IMG] Genetic studies on the secretion process in gram-negative bacteria have made considerable progress. Within the near future, such studies...
[IMG] Volume 95 provides a convenient index to the contents of Volumes 61 through 74 and Volumes 76 through 80. The nomenclature and usage in the...
[IMG] Until a decade ago an impressive amount of work had been performed in polyamine biochemistry that was based on surface-chromatographic...
[IMG] This chapter discusses the intra-lymph node technique used to inoculate rabbits with small quantities of antigen. In the experiment, 0.5 ml...
[IMG] FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein...
[IMG] This chapter describes the purification and the properties of hexokinase found in rabbit reticulocytes. This enzyme shows a marked decrease...
[IMG] This chapter focuses on the resolution of DL-myo-inositol 1-phosphate and other sugar enantiomers by gas chromatography (GC). Enantiomeric...
[IMG] This chapter lists the names of people who have contributed to the Volume 88 of the book, Methods in Enzymology, such as N. G. Abdulaev,...
[IMG] This chapter lists the names of people who have contributed to the Volume 87 of the publication, Methods in Enzymology, such as Keith...
[IMG] This chapter lists the names of people who have contributed to the Volume 86 of the publication, Methods in Enzymology, such as Ariff Ally,...
[IMG] The techniques and preparative procedures of the contractile protein chemist are becoming increasingly important to investigators whose...
[IMG] This chapter discusses the proteolytic enzymes of the complement system and pathway of the activation of complement. The activation of...
[IMG] Crystallization of a substance satisfies one of the classical criteria for homogeneity. Crystallization provides a useful purification step....
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