[IMG] Interferon (IF), the body's most rapidly produced defense against viruses, is a protein secreted by body cells when they are stimulated by...
[IMG] The collection of 14CO2 derived from appropriately labeled test compounds has several methodological advantages. The procedure is...
[IMG] This chapter deals with the preparation of blood hemoglobins of vertebrates. The hemoglobins of vertebrates are the most extensively studied...
[IMG] This book presents the second cumulative index prepared for the book “Methods in Enzymology.” It employs computer programs and procedures...
[IMG] This chapter discusses the equilibrium and kinetic inhibition assays based upon fluorescence polarization. Assays, utilizing the high...
[IMG] This chapter discusses the strategies and procedures for the preparation of monoclonal antibodies (McAb). The derivation of permanent lines...
[IMG] This chapter discusses the extraction of tissue lipids with a solvent of low toxicity. The extraction of lipids from tissues is invariably...
[IMG] The principles underlying the various assays of acetyl-CoA carboxylase have been described in previous articles in this series. H Most...
[IMG] As a pediatrician, writer, wife, and mother, Perri Klass has demonstrated how medicine is integral to the health of families and...
[IMG] The selection and use of genetic mutants to study complex biochemical problems is a well-established technique of enzymology. Selected...
[IMG] This chapter presents a directory of restriction endonucleases. The chapter presents a table that serves as a directory to the restriction...
[IMG] This chapter discusses the separation of cobyrinic acid and its biosynthetic precursors by ion-exchange paper chromatography. Cobyrinic acid...
[IMG] This chapter discusses the experimental study, focusing on the determination of nicotinamide. Nicotinamide and nicotinate are generally...
[IMG] This chapter serves as a directory to the restriction endonucleases that have not been characterized. All endonucleases which cleave DNA at...
[IMG] This chapter discusses that isotope exchange methods constitute an entire domain of enzyme kinetics that until 20 years ago was largely...
[IMG] The amount of auxiliary enzyme to be added to a assay system utilizing a single coupled reaction can be calculated from V2=−2.303 log (1 −...
[IMG] This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining...
[IMG] This chapter presents two experimental arrangements to describe the role of pulsed laser light in the ultracentrifuge: one arrangement...
[IMG] "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to...
[IMG] This chapter describes the procedures involved in the determination of a crystal structure of a tRNA by crystallographic method....
[IMG] This is an unabridged, spiral-bound versions ofCell Culture Volume 58 ofMethods in Enzymology , designed to meet the needs of the...
[IMG] This chapter presents a procedure for purification of the light-emitting system that consists of gel filtration for removal of compounds of...
[IMG] The existence of a mitochondrial genetic system has been established for only a decade, a vast amount of information about its structure,...
[IMG] This chapter provides an overview of kinetic measurements in bimolecular reactions. Kinetic methods used for measurements of reactions...
[IMG] Majority of current research on mitochondria has been performed on mitochondria obtained from rat liver, rat heart, or ox heart because...
[IMG] This chapter describes the preparation and properties of the enzymes and enzyme complexes of the mitochondrial oxidative phosphorylation...
[IMG] This chapter gives an overview of the molecular oxygen in biological oxidations. The chapter surveys the properties of the oxidases in an...
[IMG] This chapter describes the assay methodology of phosphoribosylpyrophosphate synthetase (PRPP) (ribose-5-phosphate pyrophosphokinase) from...
[IMG] Complex carbohydrates are often subjected to different chemical modifications in order to gain information on sequences and anomeric...
[IMG] This chapter provides information on the scanning calorimetry. The technique of calorimetry is the subject of an extensive review that...
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