[IMG] Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin...
[IMG] The Cumulative Subject Index for Volumes 195(198 and 200(227 includes the contents of the volumes covered, the subject index, contributor...
[IMG] This chapter discusses several methods of glycosidic bond cleavage and two major derivatization methods—formation of alditol acetates and...
[IMG] This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes...
[IMG] Although the presence of paramagnetic centers in the active site of proteins may severely limit the use of NMR for structural studies, it...
[IMG] We have seen from the previous discussion that absorption spectral studies in the ligand field region probe the energy splittings of thed...
[IMG] This chapter discusses fusion assays monitoring intermixing of aqueous contents. The fusion of phospholipid vesicles has been used as a...
[IMG] This chapter focuses on the modular design of proteases of coagulation, fibrinolysis, and complement activation—that is, its implications...
[IMG] This volume includes sections on reconstitution in cell-free extracts, reconstitution using semi-intact and perforated cells, and...
[IMG] This chapter reviews alternate methods for the generation of sequencing templates from amplified DNA and sequencing by the method of Sanger....
[IMG] Escherichia coli (E. coli) bacteria are a powerful tool for the production of heterologous proteins in large quantities, which is of general...
[IMG] This volume contains a comprehensive collection of methods for the isolation of platelet membranes, subcellular organelles, the...
[IMG] A number of methods have been employed to investigate the absorption of carotenoids in humans. The most common has been the acute or chronic...
[IMG] This volume and its companion, volume 214 of Methods in Enzymology, present a comprehensive, state-of-the-art compilation of the molecular...
[IMG] This chapter presents an overview of least-squares methods for the estimation of parameters by fitting experimental data. Least-squares...
[IMG] This volume provides up-to-date information on the purification and characterization of the enzymes involved in phospholipid biosynthesis...
[IMG] Methods widely used in the study of DNA binding proteins are presented in this volume. These include purification and protein...
[IMG] This chapter discusses the separation of phosphotyrosine, phosphoserine, and phosphothreonine by high-performance liquid chromatography...
[IMG] Protein kinases are defined as enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate...
[IMG] This chapter lists the terms mentioned in the book, Methods in Enzymology, such as abzymes, alcohol oxidase assay, carotenoids analysis, and...
[IMG] This volume includes sections dealing with specific growth factors in which new, updated, or alternative procedures are presented for...
[IMG] This volume on Phospholipases, the first of its kind, presents techniques for the assay, purification, and characterization of these...
[IMG] This volume attempts to describe the purification and assay of the diverse group of proteins involved in otoplasmic organization. Some of...
[IMG] This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent...
[IMG] Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures...
[IMG] Retinoid metabolism in the retinal pigment epithelium (RPE), with respect to the visual cycle, encompasses several steps: uptake of retinol,...
[IMG] This chapter describes the chemical synthesis of retinyl β-glucuronide (ROG) and retinoyl β-glucuronide (RAG). It discusses the extraction...
[IMG] The enzyme system from Pseudomonas oleovorans that catalyzes the hydroxylation of terminal methyl groups of alkanes and fatty acids was...
[IMG] This volume concentrates on the four major expression systems that are commonly available to most investigators: Escherichia coli, Bacillus...
[IMG] The avidin-biotin complex has been used for isolation (affinity chromatography), localization (affinity cytochemistry, cell cytometry, and...
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