[IMG] Mucin glycoproteins (mucins or MGP) are one of the principal components of the epithelial mucosal layers lining mammalian respiratory,...
[IMG] The procedures described in this chapter have enabled us to identify and characterize monoclonal antibodies and their respective...
[IMG] This volume, as does Volume 176, provides a general background of modern NMR techniques, with a specific focus on NMR techniques that...
[IMG] The nuclear overhauser effect (NOE) can be regarded as a phenomenon in nuclear magnetic resonance (NMR) measurements allowing an...
[IMG] This chapter lists the terms mentioned in the book, Methods in Enzymology, such as absorption spectroscopy nucleic acids, academia...
[IMG] This chapter discusses the measurement of transported calcium in synaptosomes. The study of Ca2+ transport across the plasma membrane of...
[IMG] The lamellar configuration of the red cell membrane includes a (liquid) superficial bilayer of amphiphilic molecules supported by a (rigid)...
[IMG] This chapter presents some general guidelines for the separation of plasma membranes as a functional entity. Any membrane isolation involves...
[IMG] This chapter provides overview transport machinery. It is central to the understanding of membrane transport to recognize that there is a...
[IMG] This chapter focuses on principles of isolation that seem likely to apply even when more demanding structural and functional tests of the...
[IMG] This chapter describes the process of isolating human platelets from plasma by centrifugation and washing. Through the centrifugation and...
[IMG] Volume 168 of Methods in Enzymology will prove invaluable not only to those in the field but also to those in related disciplines who find...
[IMG] In recent years, cyanobacteria have become the focus of intense study. As simple bacteria, they would seem to be the obvious organisms of...
[IMG] This volume includes chapters on the newest techniques for measuring metabolites of branched-chain amino acids, many new enzyme assays such...
[IMG] Microbial toxins are useful as agents in studies on membrane structure, biochemical pathways, and cellular regulatory mechanisms. Since...
[IMG] This volume includes a variety of methods involving electron microscopy and other biophysical methods, such as crystallography, neutron...
[IMG] Chemotaxis and inflammation, like as other biological processes, can be divided into humoral and cellular components. In this simplest sense...
[IMG] From the Preface: Chemotaxis and inflammation, as many other biological processes, can be divided into humoral and cellular components. In...
[IMG] This chapter presents some of the most useful methods for lignin isolation. The best known isolated lignin is probably Klason lignin, which...
[IMG] This volume covers cellulose and hemicellulose and includes proven and reproducible methods for research related to the conversion of...
[IMG] Novel approaches for the study of phosphodiesterases and phosphoprotein phosphatases, as well as those of nonmammalian cyclic nucleotide...
[IMG] The transport volumes of the Biomembranes series were initiated with Volumes 125 and 126 of Methods in Enzymology. These two volumes covered...
[IMG] Most mammalian cells have Na,K-pumps in their membranes, which pump Na ÷ ions out of and K ÷ ions into the cell, thereby creating an...
[IMG] This chapter focuses on the methodology that is used to characterize recognition sequences and on the application of HgiCI (BanI) fragment...
[IMG] This chapter describes detailed procedures for the construction of full-length complementary deoxyribo nucleic acid (cDNA) expression...
[IMG] This chapter discusses the production of single-stranded plasmid DNA. The chapter focuses on M13KO7 and its uses. The chapter reviews M13...
[IMG] This chapter focuses on the membrane transport of calcium. Ca2+ signaling plays an important role in numerous areas of cell biochemistry and...
[IMG] This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the...
[IMG] Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first...
[IMG] In this volume, in vitro models for the study of lymphoid cell functions and methods for the study of lymphoid cell receptors are presented....
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