Methods in Cell Biology Vol.17 Chromatin and Chromosomal Protein Research II

We have attempted to present, in four volumes of Methods in Cell Biology, a collection of biochemical, biophysical, and histochemical procedures that constitute the principal tools for studying eukaryotic gene expression. Contained in Volume 16 are methods for isolation of nuclei, preparation and fractionation of chromatin, fractionation and characterization of histones and nonhistone chromosomal proteins, and approaches for examining the nuclear-cytoplasmic exchange of macromolecules. This volume (Volume 17) deals with further methods for fractionation and characterization of chromosomal proteins, including DNA affinity techniques. Also contained in this volume are methods for isolation and fractionation of chromatin, nucleoli, and chromosomes. Volume 18 focuses on approaches for chromatin fractionation, examination of physical properties of chromatin, and immunological as well as sequence analysis of chromosomal proteins. In the fourth volume (Volume 19) enzymic components of nuclear proteins, chromatin transcription, and chromatin reconstitution are described. Volume 19also contains a section on methods for studying histone gene expression.

• Hardcover: 451 pages
• Publisher: Academic Press
• Language: English
• ISBN-10: 0125641176
• ISBN-13: 978-0125641173

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