Methods in Cell Biology Vol.29 Fluorescence Micoscopy of Living Cells in Culture, Part A

The first volume (Volume 29) deals with the preparation, delivery, and detection of fluorescent probes. The first half is focused on the preparation of specific structural probes, including fluorescent analogs that can be utilized by living cells in structural assembly, fluorescent molecules that bind to specific cellular components, and probes that can be used to label particular cellular compartments. There are special challenges in the preparation of each class of probes, including proteins, small peptides, heterocyclic compounds, lipids, and polysaccharides. Subsequent chapters discuss factors that determine the destination of probes and methods for delivering probes to specific sites in living cells. The second half of the first volume discusses the detection of fluorescent probes in living cells, including issues related to sample physiology (microscope cell culture), optics (basic fluorescence microscopy), and signal detection (electronic photometry and imaging, immunoelectron microscopic detection of fluorophores). The last few chapters introduce modern techniques in image detection and provide a continuity to quantitative, analytical methods covered in Volume 30.

• Hardcover: 298 pages
• Publisher: Academic Press (January 11, 1989)
• Language: English
• ISBN-10: 012564129X
• ISBN-13: 978-0125641296
• Product Dimensions: 0.8 x 6.2 x 9.5 inches

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