Methods in Cell Biology Vol.49 Methods in Plant Cell Biology, Part A

Discussion in 'Methods in Cell Biology Book Series' started by admin, Jul 10, 2016.

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

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    This chapter provides information on the scanning calorimetry. The technique of calorimetry is the subject of an extensive review that includes a complete discussion of differential heat capacity calorimetry. An interesting property of biological macromolecules is their ability to undergo structural changes with temperature. Lipids in aqueous environments undergo gel-to-liquid-crystal transitions, proteins undergo unfolding or denaturation transitions, and base-paired nucleic acids unwind. These transitions have proved to be highly cooperative in nature and are hence sharply defined. The frontier areas of modern biochemical research are more susceptible to meaningful calorimeteric investigation than has ever been the case in the past. Differential heat-capacity calorimetry has the potential for resolving all the structural transitions that a system undergoes as it is perturbed by systematic temperature variation. The study of structural domains is important, because it appears that in many instances the functional specialization arising in complicated systems occurs along the same dividing lines as those that separate structural domains.
    • Series: Methods in Cell Biology (Book 49)
    • Hardcover: 573 pages
    • Publisher: Academic Press; 1 edition
    • Language: English
    • ISBN-10: 0125641516
    • ISBN-13: 978-0125641517
    • Product Dimensions: 9.4 x 7.7 x 1.2 inches
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    Last edited: Jul 13, 2022

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