Methods in Enzymology Vol.126 Biomembranes, Part N: Transport in Bacteria, Mitochondria

Discussion in 'Methods in Enzymology Book Series' started by thuycat191992, Jul 21, 2016.

  1. thuycat191992

    thuycat191992 New Member

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    This chapter provides an overview of the historical perspectives and unifying principles of proton-motive force and secondary transport. The physiological concept and single-channel recording are described. A pump is a translocator that drives ions against their electrochemical potential gradient using energy derived from ATP or other sources, and a channel is a pore in a membrane through which a certain solute is transported down its electrochemical potential gradient. The chapter also discusses the genetic concept of the primary structure of translocators. A translocator for a single solute is a uniporter. The porters need not be intrinsically anisotropic and are required to couple the flow of pump-driven ions to the flows of specific solutes by a secondary mechanism via ion circuits across the membrane. Acid-base cluster may be an essential transmembrane structure forming an aqueous pore of acetylcholine receptor, mitochondrial porin, and many other translocators. The chapter highlights the methods for unsolved problems in molecular mechanisms.
    • Series: Methods in Enzymology (Book 126)
    • Hardcover: 856 pages
    • Publisher: Academic Press; 1 edition (May 12, 1986)
    • Language: English
    • ISBN-10: 0121820262
    • ISBN-13: 978-0121820268
    • Product Dimensions: 6 x 9 inches
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