Methods in Enzymology Vol.129 Plasma Lipoproteins, Part B: Characterization, Cell Biology

This chapter describes the application and execution of zonal ultracentrifugation for analysis, isolation, and characterization of human plasma lipoproteins and describes the major characteristics of lipoprotein particles isolated by this method. Zonal ultracentrifugation is a form of density gradient centrifugation. With this technique, macromolecules or particles are separated as discrete bands in a preformed supporting density gradient. The basis for separation is either difference in sedimentation rate or in density. The zonal centrifuge principle has become a widely used technique for separating and purifying macromolecules, subcellular organelles, and viruses. The primary advantage of zonal ultracentrifugation over other ultracentrifugation methods used in isolating lipoproteins is its superior power of resolution, its large capacity capability, but most importantly, its simultaneous analytical and preparative capabilities. This feature gives immediate information on abundance of lipoproteins and the quality of separation. It is most useful for detecting anomalous lipoproteins such as those occurring in cholestatic liver disease.

• Series: Methods in Enzymology
  
• Hardcover: 939 pages
• Publisher: Academic Press;
• Language: English
• ISBN-10: 0121820297
• ISBN-13: 978-0121820299
• Product Dimensions: 6 x 9 inches

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