Methods in Enzymology Vol.136 Immobilized Enzymes and Cells, Part C

This chapter describes general aspects related to immobilized coenzymes and also to provide a number of additional references. Coenzymes usually have to be modified to allow proper immobilization and regeneration. This modification of a coenzyme can be accomplished in basically two ways. One is the preassembly approach and the other has been called the solid-phase modular approach. In the preassembly approach, the coenzyme is first modified and then assembled with a spacer molecule to increase the steric availability for the coenzyme of the enzyme and subsequently coupled to a support. Immobilized active coenzymes, such as nicotinamide adenine dinucleotide (NAD), have found use in analytical systems as well as in enzyme reactors. The reactor of choice is the membrane reactor in which a semipermeable membrane retains the high molecular weight immobilized coenzyme together with the enzymes. However, an important issue both in the reactor systems and for analytical systems is the requirement for regeneration of the coenzyme because of its high cost and the fact that it is needed in stoichiometric amounts relative to the product formed. Regeneration can be accomplished chemically, electrochemically, or enzymatically. At present, enzymatic regeneration has the most advantages, especially because of its high-specificity in regeneration. However, electrochemical procedures are gaining in importance.

• Series: Methods in Enzymology (Book 136)
• Hardcover: 584 pages
• Publisher: Academic Press; 1 edition
• Language: English
• ISBN-10: 012182036X
• ISBN-13: 978-0121820367
• Product Dimensions: 6 x 1.3 x 9 inches

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