Methods in Enzymology Vol.137 Immobilized Enzymes and Cells, Part D

This chapter provides an introduction to immobilization techniques for enzymes. Analytical applications of immobilized enzymes and cells are probably more widespread than any other use of immobilized systems. Biosensors, in which the biocatalyst is held in close contact with the transducer, are receiving increasing attention. Several examples, presented, such as immobilization of an enzyme on or around an electrode, constitute a biosensor. Another example is the bioluminescence monitor, in which the light produced by the immobilized system is directly detected by a photosensor. The chapter describes bioselective electrodes; an enzyme electrode can be more or less complex depending on the number of enzymes utilized. Enzymes are used in anti-interference layers to remove disturbing components from a sample, in recycling systems to increase the sensitivity, and in competitive enzymatic routes to increase the applicability of, for example, a glucose sensor. The chapter also describes affinity sensors; bioaffinity can be used to increase the selectivity of a sensor in which antibody–antigen or lectin–carbohydrate binding is used to separate the analyte from a complex mixture. Detection can be accomplished via enzyme-amplified reactions.

• Series: Methods in Enzymology (Book 137)
• Hardcover: 767 pages
• Publisher: Academic Press; 1 edition
  
• Language: English
• ISBN-10: 0121820378
• ISBN-13: 978-0121820374
• Product Dimensions: 1.5 x 6.5 x 9.5 inches

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