Methods in Enzymology Vol.149 Drug and Enzyme Targeting, Part B

This chapter describes various biochemical methods for the study of receptor-mediated endocytosis. It describes the used cultured rat hepatocytes to explore the pathway involved in receptor-mediated endocytosis and catabolism of desialylated glycoproteins. This system has served as a paradigm for endocytosis those results in ligand degradation and receptor reutilization. In the course of these, studies have developed and utilized a series of assays that distinguish ligand molecules at a given stage in the pathway from those at mere proximal or distal stages. Biochemical analysis has a disadvantage compared to morphological studies in that, it is less able to pinpoint where in the cell a given process is occurring. However, with biochemical analyses, it is possible to examine an entire population of ligand molecules, thus removing somewhat the question of whether a given morphological finding is representative of the population. Examination of the entire population also facilitates kinetic modeling of ligand flux through the endocytic pathway. Finally, biochemical assays may be capable of distinguishing two stages of the process of endocytosis that exhibit indistinguishable morphology.

• Series: Methods in Enzymology
• Hardcover: 359 pages
• Publisher: Academic Press (December 12, 1987)
• Language: English
• ISBN-10: 0121820491
• ISBN-13: 978-0121820497
• Product Dimensions: 6 x 9 inches

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