Methods in Enzymology Vol.158 Metallobiochemistry, Part A

This chapter focuses on the membrane transport of calcium. Ca2+ signaling plays an important role in numerous areas of cell biochemistry and physiology, ranging from exocrine and hormonal secretion, to muscle and nonmuscle motility, to the activity and regulation of several important metabolic pathways. The central element in the signaling function of Ca2+ is its reversible complexation by specific proteins, which bind Ca2+ in micromolar or submicromolar concentrations with optimal affinity in the presence of much larger concentrations of other divalent and monovalent cations, such as Mg2+, Na+, and K+. Many of these proteins are dissolved in the cytosol or organized in nonmembrane structures, such as the myofibrils. Interest in endoplasmic reticulum as a Ca2+ reservoir has been heightened by the finding that inositol trisphosphate, one of the two important catabolites of phosphatidylinositol diphosphate, specifically releases Ca2+ from endoplasmic reticulum in response to a number of plasma membrane stimuli that activate a phospholipase. The release was originally demonstrated in permeabilized exocrine pancreas cells, but more recently, has been shown in isolated endoplasmic reticulum vesicles. In the latter system, however, particular conditions must be applied.

• Series: Methods in Enzymology
  
• Hardcover: 464 pages
• Publisher: Academic Press;
  
• Language: English
• ISBN-10: 0121820599
• ISBN-13: 978-0121820596
• Product Dimensions: 6 x 1.1 x 9 inches

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