Methods in Enzymology Vol.161 Biomass, Part B: Lignin, Pectin, and Chitin

This chapter presents some of the most useful methods for lignin isolation. The best known isolated lignin is probably Klason lignin, which is obtained by treating wood with sulfuric acid. The polysaccharides are hydrolyzed to water-soluble sugars, and the lignin is recovered as an insoluble residue. Although this method for lignin isolation has great utility as an analytical means of determining lignin content, the highly condensed and altered Klason lignin is generally unsuited for either chemical characterization or studies of biological modification and degradation. The most useful lignin preparation is Björkman lignin, also known as Björkman milled wood lignin or simply milled wood lignin (MWL). Milled wood lignin is purified from the aqueous p-dioxane extract of finely milled wood, which has been first extracted with organic solvents to remove extraneous components. Although it has not been rigorously proved that MWL is representative of protolignin, it is considered to be appropriate for most chemical and biological studies. The chapter discusses those procedures used to isolate lignin from wood.

• Series: Methods in Enzymology
  
• Hardcover: 574 pages
• Publisher: Academic Press
• Language: English
• ISBN-10: 0121820629
• ISBN-13: 978-0121820626
• Product Dimensions: 6 x 1.3 x 9 inches

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