Methods in Enzymology Vol.172 Biomembranes, Part S Transport Membrane Isolation and Characterization

This chapter presents some general guidelines for the separation of plasma membranes as a functional entity. Any membrane isolation involves three successive steps: (1) tissue homogenization, (2) fractionation, and (3) analysis. In science, a precise and unambiguous vocabulary is necessary to avoid the misinterpretation of procedures and/or results. Homogenization is the reduction of a sample to evenly distributed particles of uniform sizes; it is the disruption of a cell envelope under conditions where the integrity of cellular organelles—such as nuclei, mitochondria, and lysosomes—is maintained. While comparing the degrees of purification, a specific activity of marker enzymes in the final membrane fractions, together with the enrichment factors and the enzyme recoveries, should be considered. Thus, the structural integrity and the biochemical integrity of different cellular components are the prerequisites for a successful fractionation, isolation, and final analysis of homogeneous membrane preparations.

• Series: Methods in Enzymology
  
• Hardcover: 776 pages
• Publisher: Academic Press
• Language: English
• ISBN-10: 0121820734
• ISBN-13: 978-0121820732
• Product Dimensions: 6 x 1.7 x 9 inches

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