Methods in Enzymology Vol.174 Biomembranes, Part U: Cellular and Subcellular Transport

This chapter discusses the measurement of transported calcium in synaptosomes. The study of Ca2+ transport across the plasma membrane of isolated nerve terminals (synaptosomes) and the subsequent distribution of the cation among the various subsynaptosomal compartments is central to the investigation of the Ca2+ dependency of neurotransmitter release. Calcium transport in synaptosomes may serve as a model for the integration of cellular Ca2+ transporting pathways in general. The mitochondria are major stores of Ca2+ within the isolated synaptosomes. To determine the proportion of the transported Ca2+ that is further translocated into the intrasynaptosomal mitochondria, a rapid disruption of the synaptosomes is carried out using a combination of digitonin and shear under conditions that minimize any artifactual redistribution of Ca2+ across the mitochondrial membrane. The chapter concludes with the discussion of Ca2+ cycling across the synaptosomal plasma membrane.

• Series: Methods in Enzymology
  
• Hardcover: 725 pages
• Publisher: Academic Press
• Language: English
• ISBN-10: 0121820750
• ISBN-13: 978-0121820756
• Product Dimensions: 6 x 9 inches

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