Methods in Enzymology Vol.176 Nuclear Magnetic Resonance, Part A: Spectral Techniques and Dynamics

The nuclear overhauser effect (NOE) can be regarded as a phenomenon in nuclear magnetic resonance (NMR) measurements allowing an experimenter to derive estimates of internuclear distances in molecules as well as information about rotational correlation times. The NOE factor, which was originally determined by the saturation of a neighboring spin with an rf field, is defined as a measure of change in the steady-state description of a nuclear spin system resulting from an external perturbation. There are a number of alternative techniques available for the measurement of NOE factors. The classical one-dimensional technique uses the difference in peak areas as, in which the reference spectrum is measured in the absence of saturating irradiation, or is measured in a region in which there are no resonances. The aim of any experiment is to measure parameters as accurately as possible within a reasonable amount of time. From an experimental point of view, one must pay careful attention to the effects of the rf field on intensity measurements, because it is possible to overload the spectrometer preamplifier and detection system, and because it is possible to partially saturate the resonances giving rise to the peaks. It is impossible to suggest an algorithm for minimizing the effects of systematic errors in peak intensity measurements.

• Series: Methods in Enzymology
  
• Hardcover: 530 pages
• Publisher: Academic Press;
  
• Language: English
• ISBN-10: 0121820777
• ISBN-13: 978-0121820770
• Product Dimensions: 6 x 1.2 x 9 inches

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