Mucin glycoproteins (mucins or MGP) are one of the principal components of the epithelial mucosal layers lining mammalian respiratory, gastrointestinal, and reproductive tracts. The procedures described in this chapter focus on isolation and characterization of human tracheobronchial mucins (TBM). The protocols are useful for normal and bronchial asthmatic mucus. Modifications may be required for isolating TBM from lung mucosal gel (LM) gel samples of individuals with cystic fibrosis. Isolation of TBM by density centrifugation has been examined in some detail. This chapter has demonstrated that while CsBr is more effective for solubilizing lung mucus than CsCl, density centrifugation in CsBr does not adequately separate mucins from proteins. Recent studies indicate that only trace amounts of lipids are present after exhaustive delipidation of mucins, including TBM. Series: Methods in Enzymology (Book 179) Hardcover: 639 pages Publisher: Academic Press; 1 edition (January 11, 1990) Language: English ISBN-10: 0121820807 ISBN-13: 978-0121820800 Product Dimensions: 1.2 x 6.8 x 9.8 inches Shipping Weight: 2.3 pounds Link Download http://nitroflare.com/view/1DE6838B9F1C4D6https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
