This chapter discusses the strategies for obtaining mutants in the bacteria Escherichia coli and Salmonella typhimurium. The heights of accomplishment in bacterial or bacteriophage genetics have usually been ingenious genetic schemes for isolating mutants or for determining the basis of some biological phenomenon. One of the most striking examples of exploits in this field is the use of frameshift mutations to determine the triplet nature of the genetic code. The strategy of the geneticist is to look for mutants affecting a particular biological process for using them to understand that process. Genetics are necessary either to show that some biochemical finding has a reality in the living cell or to determine the nature of the biochemical process in the first place. The current ability to do reverse genetics has heightened awareness of the importance of the genetic approach. Series: Methods in Enzymology Hardcover: 706 pages Publisher: Academic Press Language: English ISBN-10: 0121821056 ISBN-13: 978-0121821050 Product Dimensions: 6 x 1.6 x 9 inches Link Download http://nitroflare.com/view/1A5084ABAEB52F2https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
