Methods in Enzymology Vol.214 Carotenoids, Part B: Metabolism, Genetics, and Biosynthesis

A number of methods have been employed to investigate the absorption of carotenoids in humans. The most common has been the acute or chronic dosing of a carotenoid, such as β-carotene, and the measurement of the plasma response. The gastrointestinal lavage technique, wherein the entire gastrointestinal tract of the subject is cleansed of ingesta prior to consumption of the compound or food component whose absorption is being measured, has been reported for assessing the absorption of calcium but more recently was applied for measuring carotenoid absorption in humans. Metabolic balances can provide estimations of the apparent disappearance of various carotenoids from the gut, and therefore provide a crude estimation of the amount absorbed under various experimental conditions, assuming no degradation or microbial synthesis within the gut. The simultaneous measurement of various tissue responses provides valuable information in conjunction with these techniques. The availability of stable isotopes of various carotenoids holds great promise to provide definitive data in the characterization and quantitation of carotenoid absorption in humans.

• Series: Methods in Enzymology (Book 214)
• Hardcover: 468 pages
• Publisher: Academic Press; 1 edition (April 6, 1993)
• Language: English
• ISBN-10: 0121821153
• ISBN-13: 978-0121821159
• Product Dimensions: 1 x 6.5 x 9.5 inches

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