Methods in Enzymology Vol.220 Membrane Fusion Techniques, Part A

This chapter discusses fusion assays monitoring intermixing of aqueous contents. The fusion of phospholipid vesicles has been used as a model system for understanding the molecular mechanisms of membrane fusion in biological systems. Fusion is monitored by a variety of techniques, including differential scanning calorimetry, dynamic light scattering, and gel filtration. These techniques essentially measure changes in the system under study before and after the induction of fusion. Methods to study the kinetics of the fusion process include measuring the dilution of spin-labeled phospholipids from labeled to unlabeled vesicles as an indicator of lipid mixing, monitoring the intermixing or dilution of fluorescent phospholipids, and following the intermixing of aqueous contents of vesicles. The processes taking place during membrane fusion are (1) adhesion or aggregation of two membranes or vesicles, (2) establishment of molecular contact between the membranes, (3) membrane destabilization at the area of intermembrane contact, (4) merging of membrane components, and (5) coalescence of the internal aqueous contents of the vesicles or compartments bounded by the membranes.

• Series: Methods in Enzymology (Book 220)
• Hardcover: 433 pages
• Publisher: Academic Press; 1 edition (July 1, 1993)
• Language: English
• ISBN-10: 0121821218
• ISBN-13: 978-0121821210
• Product Dimensions: 6 x 1 x 9 inches

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