Methods in Enzymology Vol.230 Guide to Techniques in Glycobiology

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  1. PrettyYang

    PrettyYang New Member

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    This chapter discusses several methods of glycosidic bond cleavage and two major derivatization methods—formation of alditol acetates and trimethylsilylation (TMS)—used for glycosyl composition analysis. Basic procedures for cleavage of oligosaccharides and for the derivatization methods used for composition analysis are also described. One of the methods of cleavage is methanolysis. Glycosidic bonds differ in their susceptibility to acid hydrolysis. The rate of release is dependent on the position and anomeric configuration of the glycosidic linkage and on the identity of the monosaccharide. An alternate method of cleavage is methanolysis. This method results in high yields of the carbohydrates from glycoproteins and from other glycoconjugates because there is less destruction of carbohydrate than with aqueous acids. In the TMS derivatization reaction, the monosaccharide hydroxyls are converted to silyl ethers. This modification occurs quickly under mild conditions converting the monosaccharides to volatile derivatives suitable for gas–liquid chromatography. The TMS method allows the simultaneous identification of neutral sugars and amino sugars and of acidic sugars.
    • Series: Methods in Enzymology (Book 230)
    • Hardcover: 567 pages
    • Publisher: Academic Press; 1 edition (January 17, 1994)
    • Language: English
    • ISBN-10: 0121821315
    • ISBN-13: 978-0121821319
    • Product Dimensions: 1 x 6.5 x 9.5 inches
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