This volume presents the best techniques and strategies for the study of lipid modified proteins, with particular emphasis on methods which evaluate the functional significance or biological impact of lipid modification. Included are the biological methods for the study of function (yeast genetics; cloning strategies; mutational analysis; expression systems), biochemical methods for the study and purification of enzymes or modified proteins (in vitro assays using peptide, native, or recombinant protein substrates; coupled in vitro cDNA transcription, translation/modification; bacolovirus expression; lipid analogs/inhibitors); physical methods for the identification of lipid groups (cleavage techniques; modification techniques; simple separations: TLC, GC, HPLC, ES/MS, tandem MS). Series: Methods in Enzymology (Book 250) Hardcover: 754 pages Publisher: Academic Press; Language: English ISBN-10: 012182151X ISBN-13: 978-0121821517 Product Dimensions: 9.3 x 6.3 x 1.6 inches Link Download http://nitroflare.com/view/D4A5603FF547897https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
