Methods in Enzymology Vol.264 Mitochondrial Biogenesis and Genetics Part B

In vivo footprinting is a very useful technique to obtain information on the functional behavior of mitochondrial DNA (mtDNA)-binding proteins, not only when studying normal cells subjected to different environmental conditions or grown under different physiological situations, but also in the analysis of some disease-causing mutations affecting regulatory sequences of mtDNA. This chapter describes a protocol that has been successfully applied in the laboratory to the in vivo study of the interactions between mitochondrial transcription termination factor (mTERF) and mtDNA and to the investigation of protein–DNA interactions in the regions of the H-strand and L-strand transcription promoters. The simplicity and rapidity of the method and the limited number of cells required for an experiment make the protocol the method of choice to study protein–DNA interactions involving regulatory sequences of mtDNA for understanding their role in transcription and replication.

• Series: Methods in Enzymology (Book 264)
• Hardcover: 621 pages
• Publisher: Academic Press; 1 edition (March 22, 1996)
• Language: English
• ISBN-10: 012182165X
• ISBN-13: 978-0121821654
• Product Dimensions: 1.2 x 6.5 x 9.5 inches

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