Methods in Enzymology Vol.267 Combinatorial Chemistry Part A

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

    When the fitness being selected for is affinity for a target receptor molecule, the foregoing program is called “affinity maturation,” the term coined by immunologists for the interspersed rounds of selective stimulation by antigen and somatic mutation of antibody genes that is thought to give rise to antibodies, with increasing affinity in the course of an immune response. This chapter discusses the affinity maturation from random peptide libraries displayed on phage. The procedures and underlying principles are discussed in the context of a specific exemplar experiment, in which ligands for a model receptor were selected from a library of random 15-mers. The model receptor is S-protein, a 104-residue fragment of bovine ribonuclease prepared by partial digestion, with subtilisin; the other fragment, S-peptide, corresponds to the N-terminal 20 amino acids. Neither fragment alone is enzymatically active, but when they are mixed S-peptide binds strongly to S-protein, restoring the enzyme activity.
    • Series: Methods in Enzymology (Book 267)
    • Hardcover: 493 pages
    • Publisher: Academic Press; 1 edition (May 31, 1996)
    • Language: English
    • ISBN-10: 0123693942
    • ISBN-13: 978-0121821685
    • ASIN: 0121821684
    • Product Dimensions: 1 x 6.2 x 9.5 inches
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    Last edited: Jan 14, 2022

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