Methods in Enzymology Vol.300 Oxidants and Antioxidants, Part B

Discussion in 'Methods in Enzymology Book Series' started by admin, Aug 2, 2016.

  1. admin

    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

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    This chapter has outlined methods to assess lipid peroxidation associated with oxidant injury in vivo by quantifying concentrations of free F2-IsoPs in biological fluids and levels of F2-IsoPs esterified in tissue lipids. The mass spectrometric assay described herein is highly precise and accurate. A potential shortcoming with this approach is that it requires expensive instrumentation, i.e., a mass spectrometer. However, several immunoassays for an F2-IsoP, 8-iso-PGF2α, have become available from commercial sources. At this time, the accuracy and reliability of these assay for quantifying F2-IsoPs in biological fluids has not been fully validated by mass spectrometry. If these immunoassays prove to be a reliable measure of F2-IsoPs, however, this should greatly expand the use of F2-IsoPs to assess oxidant stress.
    • Series: Methods in Enzymology (Book 300)
    • Hardcover: 530 pages
    • Publisher: Academic Press;
    • Language: English
    • ISBN-10: 012182201X
    • ISBN-13: 978-0121822019
    • Product Dimensions: 1.2 x 6.5 x 9.5 inches
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    Last edited: Feb 28, 2022

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