Methods in Enzymology Vol.300 Oxidants and Antioxidants, Part B

Discussion in 'Methods in Enzymology Book Series' started by admin, Aug 2, 2016.

  1. admin

    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

    This chapter has outlined methods to assess lipid peroxidation associated with oxidant injury in vivo by quantifying concentrations of free F2-IsoPs in biological fluids and levels of F2-IsoPs esterified in tissue lipids. The mass spectrometric assay described herein is highly precise and accurate. A potential shortcoming with this approach is that it requires expensive instrumentation, i.e., a mass spectrometer. However, several immunoassays for an F2-IsoP, 8-iso-PGF2α, have become available from commercial sources. At this time, the accuracy and reliability of these assay for quantifying F2-IsoPs in biological fluids has not been fully validated by mass spectrometry. If these immunoassays prove to be a reliable measure of F2-IsoPs, however, this should greatly expand the use of F2-IsoPs to assess oxidant stress.
    • Series: Methods in Enzymology (Book 300)
    • Hardcover: 530 pages
    • Publisher: Academic Press;
    • Language: English
    • ISBN-10: 012182201X
    • ISBN-13: 978-0121822019
    • Product Dimensions: 1.2 x 6.5 x 9.5 inches
    Link Download
    Last edited: Feb 28, 2022

Share This Page