Methods in Enzymology Vol.327 Applications of Chimeric Genes and Hybrid Proteins, Part B

The modern biologist takes almost for granted the rich repertoire of tools currently available for manipulating virtually any gene or protein of interest. Paramount among these operations is the construction of fusions. The fact that gene fusions can create functional chimeric proteins has been demonstrated shortly thereafter. From that time, the number of tricks for splicing or inserting into a gene product various markers, tags, antigenic epitopes, structural probes, and other elements has increased explosively. Hence, it became clear early on that given the huge number of applications of gene fusion and hybrid protein technology-for studies of the regulation of gene expression, for lineage tracing, for protein purification and detection, for analysis of protein localization and dynamic movement, and a plethora of other uses. This chapter focuses on procedures for tagging proteins for immunodetection, for using chimeric proteins for cytological purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and manipulating various aspects of cell signaling and cell physiology. It also discusses the green fluorescent protein (GFP).

• Series: Methods in Enzymology (Book 327)
• Hardcover: 712 pages
• Publisher: Academic Press;
  
• Language: English
• ISBN-10: 0121822281
• ISBN-13: 978-0121822286
• Product Dimensions: 1.5 x 6.2 x 9.5 inches

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