Methods in Enzymology Vol.331 Hyperthermophilic Enzymes, Part B

Within central metabolism, the essentially invariant chemistry of the citric acid cycle across all organisms supports the proposal that it was one of the first pathways to have evolved. Consequently, it is an excellent source of enzymes for comparative evolutionary and structural studies. Different organisms use the cycle for different purposes, which determine the levels of citrate synthase found in cell extracts. The anaerobic hyperthermophile, Pyrococcus furiosus, probably uses the citric acid cycle only for biosynthetic purposes and a low level of enzyme (<10 units/mg) is expected and is routinely found at the same assay temperature. This article concentrates on those methodologies that have been developed in this area and discusses both the difficulties of assaying enzymes at high temperatures using thermolabile substrates and the techniques used to characterize the catalytic and stability properties of archaeal citrate synthases.

• Series: Methods in Enzymology (Book 331)
• Hardcover: 494 pages
• Publisher: Academic Press
• Language: English
• ISBN-10: 012182232X
• ISBN-13: 978-0121822323
• Product Dimensions: 6 x 1.1 x 9 inches

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