Methods in Enzymology Vol.333 Regulators and Effectors of Small GTPases, Part G

This chapter describes the involvement of Shc proteins in signaling pathways. Shc proteins are integral components in the action of a wide variety of receptors including receptor tyrosine kinases (RTKs), G protein-coupled receptors (GPCRs), immunoglobulin receptors, and integrins. Activation of each of these receptors can lead to the recruitment of Shc proteins, cuhninating in their tyrosine phosphorylation. To date, three mammalian Shc genes are identified: ShcA, ShcB (Sck), and ShcC (N-Shc/Rai). All three Shc genes encode proteins that are highly related in sequence and structure, consisting of a carboxy-terminal Src homology 2 (SH2) domain, a central effector region rich in proline and glycine residues and containing two distinct sites for tyrosine phosphorylation (CHI), and an aminoterminal phosphotyrosine-binding (PTB) domain. The chapter also reveals three regions of the CH1 domain that are highly conserved in mammalian Shc family members. In contrast to the more carboxy-terminal tyrosine phosphorylation site, there are a number of additional amino acids surrounding the amino-terminal phosphorylation site that are also conserved among Shc family members, suggesting that these residues play an important role in Shc function through the recognition of effector proteins.

• Series: Methods in Enzymology (Book 333)
• Hardcover: 400 pages
• Publisher: Academic Press; 1 edition (June 8, 2001)
• Language: English
• ISBN-10: 0121822346
• ISBN-13: 978-0121822347
• Product Dimensions: 0.8 x 6.5 x 9.2 inches

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