Affinity labeling by definition applies to all molecules possessing a site that binds another molecule with a certain degree of specificity and affinity. In biology the proteins are the obvious ligand-binding molecules, and in practice the affinity labeling techniques have primarily involved proteins. The complete understanding of how proteins carry out their biological function requires a detailed understanding of how this binding site is constituted, and this has become the key problem in the area of protein structure-function analysis. The purpose of this chapter is to present a descriptive overview of the current status of affinity labeling; the evolution of the concepts and techniques, the most general picture of the principles involved, the terminology and the criteria commonly applied, and the current and prospective applications of affinity labeling techniques. In this chapter the discussion is kept general and is based on hypothetical examples. The chapter explains the principles and types of reagents, the Covalent Bond formation, interpretations, variations and special considerations and the use of affinity labeling. Series: Methods in Enzymology (Book 46) Hardcover: 774 pages Publisher: Academic Press; Language: English ISBN-10: 0121819469 ISBN-13: 978-0121819460 Product Dimensions: 6 x 9 inches Link Download http://nitroflare.com/view/390A87825E143DFhttps://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
