The detection and quantitative analysis of amino acids in subnanomole quantities can be accomplished by several methods. Thin-layer chromatography (TLC) has been used to detect PTH-derivatives at subnanomole levels. Combined with autoradiography, TLC techniques have been described for subpicomole levels of tritiated dansyl amino acids. Gas chromatography of amino acid derivatives has been used to analyze amino acids in picomole range. High-performance liquid chromatography of amino acid derivatives, although developed primarily for monitoring amino acid sequencing, offers a sensitive method for amino acid analysis from protein and peptide hydrolysates. When amino acid derivatives must be synthesized prior to analysis, it is necessary to ensure quantitative and reproducible derivative yields, as well as derivative stability. By carrying reagent blanks and low-level standard amino acid mixtures through the hydrolysis procedures, it is possible to correct the contamination effects and losses during hydrolysis. Series: Methods in Enzymology Hardcover: 668 pages Publisher: Academic Press Language: English ISBN-10: 0121819477 ISBN-13: 978-0121819477 Product Dimensions: 6 x 1.4 x 9 inches Link Download http://nitroflare.com/view/CA7FA06F47D7C95https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
