Methods in Enzymology Vol.47 Enzyme Structure, Part E

Discussion in 'Methods in Enzymology Book Series' started by admin, Jul 18, 2016.

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

    The detection and quantitative analysis of amino acids in subnanomole quantities can be accomplished by several methods. Thin-layer chromatography (TLC) has been used to detect PTH-derivatives at subnanomole levels. Combined with autoradiography, TLC techniques have been described for subpicomole levels of tritiated dansyl amino acids. Gas chromatography of amino acid derivatives has been used to analyze amino acids in picomole range. High-performance liquid chromatography of amino acid derivatives, although developed primarily for monitoring amino acid sequencing, offers a sensitive method for amino acid analysis from protein and peptide hydrolysates. When amino acid derivatives must be synthesized prior to analysis, it is necessary to ensure quantitative and reproducible derivative yields, as well as derivative stability. By carrying reagent blanks and low-level standard amino acid mixtures through the hydrolysis procedures, it is possible to correct the contamination effects and losses during hydrolysis.
    • Series: Methods in Enzymology
    • Hardcover: 668 pages
    • Publisher: Academic Press
    • Language: English
    • ISBN-10: 0121819477
    • ISBN-13: 978-0121819477
    • Product Dimensions: 6 x 1.4 x 9 inches
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    Last edited: Dec 5, 2021

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