Methods in Enzymology Vol.56 Biomembranes, Part G - Bioenergetics: Biogenesis of Mitochondria

The existence of a mitochondrial genetic system has been established for only a decade, a vast amount of information about its structure, function, and organization has already accumulated. Several factors account for the rapid progress in the analysis of the mitochondrial genome. The isolation of several classes of mutants affecting the mitochondrial genome has contributed greatly to the exponential growth of this field. The electron transport and energy-transducing functions of the inner mitochondrial membrane have been studied intensively for several decades, and the individual polypeptide components of the oligomeric electron transport and coupling complexes. The chapter reviews that the inner mitochondrial membrane, composed of gene products of both mitochondrial and nuclear genomes, represents an excellent system for the study of membrane assembly, because the powerful genetic approach can be applied to the assembly of a membrane having established function and composition. Research on mitochondria has progressed dramatically in the past few years from an emphasis on simple verification of the presence of distinct mitochondrial DNA to the sophisticated molecular and genetic studies, as summarized in this chapter. Even without the introduction of new techniques, application of methods currently available should lead to a far more thorough understanding of the mitochondrion as a semiautonomous genetic and protein-synthesizing element, as well as the site of oxidative phosphorylation and electron transport, within the eukaryotic cell.

• Series: Methods in Enzymology (Book 56)
• Hardcover: 832 pages
• Publisher: Academic Press;
  
• Language: English
• ISBN-10: 0121819566
• ISBN-13: 978-0121819569
• Product Dimensions: 6 x 9 inches

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