Methods in Enzymology Vol.62 Vitamins and Coenzymes, Part D

Discussion in 'Methods in Enzymology Book Series' started by admin, Jul 19, 2016.

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

    This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.
    • Series: Methods in Enzymology (Book 62)
    • Hardcover: 616 pages
    • Publisher: Academic Press
    • Language: English
    • ISBN-10: 0121819620
    • ISBN-13: 978-0121819620
    • Product Dimensions: 6 x 9 inches
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    Last edited: Dec 14, 2021

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