Methods in Enzymology Vol.65 Nucleic Acids, Part I

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

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    This chapter serves as a directory to the restriction endonucleases that have not been characterized. All endonucleases which cleave DNA at a specific sequence have been considered to be restriction enzymes, although in most cases there is no direct genetic evidence for the presence of a host-controlled restriction-modification system. The chapter presents a table—the source of each microorganism is given either as an individual or a National Culture Collection. The enzymes are named in accordance with the proposal of Smith and Nathans. The recognition sequences are abbreviated so that only one strand, reading 5' → 3', is indicated and the point of cleavage, when known, is indicated by an arrow (↓). When two bases appear in parentheses, either one may appear at that position within the recognition sequence. Where known, the base modified by the corresponding methylase is indicated by an asterisk. When more than one reference appears, the first contains the purification procedure for the restriction enzyme, the second concerns its recognition sequence, the third contains the purification procedure for the methylase, and the fourth describes its recognition sequence. In some cases two references appear in one of these categories when two independent groups have reached similar conclusions.
    • Series: Methods in Enzymology
    • Hardcover: 968 pages
    • Publisher: Academic Press; 1 edition (May 12, 1980)
    • Language: English
    • ISBN-10: 0121819655
    • ISBN-13: 978-0121819651
    • Product Dimensions: 6 x 9 inches
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    Last edited: Nov 21, 2021

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