Methods in Enzymology Vol.72 Lipids, Part D

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

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    This chapter discusses the extraction of tissue lipids with a solvent of low toxicity. The extraction of lipids from tissues is invariably done with volatile organic solvents, most of which are sold with warnings against excessive inhalation. For each gram of tissue, add 18 ml of extraction solvent and homogenize thoroughly. The mixture should be a well-dispersed suspension. After 30–60 s of mixing, filter the mixture, preferably with a sintered-glass, Buchner funnel under pressure rather than with a vacuum. Materials containing above-average water content, such as plasma, should be extracted with a larger volume of extraction solvent to keep the water in solution. In the case of plasma and whole blood, it is best to add the material to the solvent in small portions with continuous vortexing, if a fine suspension of the nonlipid portion is to be produced. The washing step is a convenient way to reduce the volume of the lipid extract, a factor to consider in working on a large scale. It removes primarily the higher boiling component, and thereby facilitates subsequent evaporative removal of solvent. Removal of solvent from hexane-isopropanol extracts is readily done by vacuum evaporation, but a warmer bath than usual is recommended.
    • Hardcover: 842 pages
    • Publisher: Academic Press;
    • Language: English
    • ISBN-10: 012181971X
    • ISBN-13: 978-0121819729
    • ASIN: 0121819728
    • Product Dimensions: 6 x 9 inches
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