This chapter deals with the preparation of blood hemoglobins of vertebrates. The hemoglobins of vertebrates are the most extensively studied of any proteins, largely because of their ease of preparation. The red cells are easily separated from the plasma by centrifugation. Hemolysis leads immediately to a solution that is usually more than 90% pure. Nevertheless, precautions need to be taken to obtain preparations of maximum value. Hemoglobins from different organisms vary greatly in their stability and resistance to denaturation, tendency to oxidize to methemoglobin, solubility, and chromatographic and electrophoretic properties. What is appropriate for mammalian hemoglobins is frequently inappropriate for those of lower vertebrates. The chapter explains erythrocyte preparation. Blood from small animals is usually best obtained by cardiac puncture. Large animals can be bled from superficial veins—for example, those of the ears of rabbits and elephants. The caudal vein of fish is an excellent location for bleeding; it is usually easy, with a little practice, to insert a needle just ventral to the lateral line directly into the vein. The extraction of hemoglobin from very small animals and embryos presents special problems. The chapter also describes the preparation of hemolysate. Series: Methods in Enzymology (Book 76) Hardcover: 912 pages Publisher: Academic Press; 1 edition (January 11, 1982) Language: English ISBN-10: 0121819760 ISBN-13: 978-0121819767 Product Dimensions: 6 x 9 inches Link Download http://nitroflare.com/view/384CE1D8275EEC7https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
