This chapter focuses on the resolution of DL-myo-inositol 1-phosphate and other sugar enantiomers by gas chromatography (GC). Enantiomeric amino acids, 2-amino-l-phenylethanols, and lactic acid are separated as simple derivatives by GC on a glass capillary column coated with a chiral liquid phase. The phase consists of N-t-butyl-L-valinamide in α-amide linkage with a copolymer of dimethylsiloxane and a carboxyalkyl-methylsiloxane. The chapter discusses the derivation procedures for trimethylsilyl-methylphosphate (TMS-Me), heptafluorobutyric (HFB) esters, and cyclic alkaneboronic esters. TMS-Me derivatives of inositol phosphates are prepared by complete trimethylsilylation of the molecules, followed by the methanolysis of the phosphate trimethylsilyl (TMS) ester moiety and then reaction with diazomethane. The Chirasil-Val column is also suitable for GC-MS. The original applications of Chirasil-Val are with nitrogen-containing substances and stressed the role of the nitrogen atoms in the achievement of chiral separations. DL-Glucose is best separated on a 20-meter column as the methaneboronate coderivatized with a trimethylsilyl group or as the butaneborcnate coderivatized with TMS or HFB moiety. Series: Methods in Enzymology (Book 89) Hardcover: 688 pages Publisher: Academic Press Language: English ISBN-10: 0121819892 ISBN-13: 978-0121819897 Product Dimensions: 6 x 1.4 x 9 inches Link Download http://nitroflare.com/view/E6E33E47B2743C4https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
