Methods in Enzymology Vol.89 Carbohydrate Metabolism, Part D

Discussion in 'Methods in Enzymology Book Series' started by admin, Jul 20, 2016.

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    admin Thư Viện Sách Việt Staff Member Quản Trị Viên

    This chapter focuses on the resolution of DL-myo-inositol 1-phosphate and other sugar enantiomers by gas chromatography (GC). Enantiomeric amino acids, 2-amino-l-phenylethanols, and lactic acid are separated as simple derivatives by GC on a glass capillary column coated with a chiral liquid phase. The phase consists of N-t-butyl-L-valinamide in α-amide linkage with a copolymer of dimethylsiloxane and a carboxyalkyl-methylsiloxane. The chapter discusses the derivation procedures for trimethylsilyl-methylphosphate (TMS-Me), heptafluorobutyric (HFB) esters, and cyclic alkaneboronic esters. TMS-Me derivatives of inositol phosphates are prepared by complete trimethylsilylation of the molecules, followed by the methanolysis of the phosphate trimethylsilyl (TMS) ester moiety and then reaction with diazomethane. The Chirasil-Val column is also suitable for GC-MS. The original applications of Chirasil-Val are with nitrogen-containing substances and stressed the role of the nitrogen atoms in the achievement of chiral separations. DL-Glucose is best separated on a 20-meter column as the methaneboronate coderivatized with a trimethylsilyl group or as the butaneborcnate coderivatized with TMS or HFB moiety.
    • Series: Methods in Enzymology (Book 89)
    • Hardcover: 688 pages
    • Publisher: Academic Press
    • Language: English
    • ISBN-10: 0121819892
    • ISBN-13: 978-0121819897
    • Product Dimensions: 6 x 1.4 x 9 inches
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    Last edited: Dec 8, 2021

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