Methods in Molecular Biology Vol.1188 Stable Isotope Labeling by Amino Acids in Cell Culture

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups.These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 1188)
• Hardcover: 372 pages
• Publisher: Humana Press; 2014 edition (July 25, 2014)
• Language: English
• ISBN-10: 1493911414
• ISBN-13: 978-1493911417
• Product Dimensions: 7 x 0.9 x 10 inches

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