Methods in Molecular Biology Vol.1258 Insoluble Proteins Methods and Protocols

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 1258)
• Hardcover: 425 pages
• Publisher: Humana Press
• Language: English
• ISBN-10: 1493922041
• ISBN-13: 978-1493922048
• Product Dimensions: 7 x 1 x 10 inches

Link Download
http://nitroflare.com/view/16624ECA90810DB
https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1