This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Series: Methods in Molecular Biology (Book 1275) Hardcover: 216 pages Publisher: Humana Press; Language: English ISBN-10: 1493923641 ISBN-13: 978-1493923649 Product Dimensions: 7 x 0.6 x 10 inches Link Download http://nitroflare.com/view/CBAE50454183C36https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1