Methods in Molecular Biology Vol.1322 Hedgehog Signaling Protocols 2nd Edition

The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogenous Gli proteins. The final chapters talk about metabolic effects of noncanonical Hh signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 1322)
• Hardcover: 200 pages
• Publisher: Humana Press; 2nd ed. 2015 edition (July 21, 2015)
• Language: English
• ISBN-10: 149392771X
• ISBN-13: 978-1493927715
• Product Dimensions: 7.2 x 0.7 x 10.4 inches

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