Methods in Molecular Biology Vol.1330 Cell Reprogramming Methods and Protocols

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells, and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 1330)
• Hardcover: 271 pages
• Publisher: Humana Press; 1st ed. 2015 edition (December 2, 2015)
• Language: English
• ISBN-10: 1493928473
• ISBN-13: 978-1493928477
• Product Dimensions: 10.4 x 7.1 x 0.8 inches

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