Methods in Molecular Biology Vol.1350 Baculovirus and Insect Cell Expression Protocols 3rd Edition

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 1350)
• Hardcover: 508 pages
• Publisher: Humana Press
• Language: English
• ISBN-10: 1493930427
• ISBN-13: 978-1493930425
• Product Dimensions: 7 x 1.1 x 10 inches

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