Methods in Molecular Biology Vol.1373 The Genetic Manipulation of Staphylococci Methods and Protocol

This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

• Series: Methods in Molecular Biology (Book 1373)
• Hardcover: 186 pages
• Publisher: Humana Press;
  
• Language: English
• ISBN-10: 1493931571
• ISBN-13: 978-1493931576
• Product Dimensions: 7 x 0.5 x 10 inches

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