A collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization; for studying the physiological roles for endogenous G proteins; and for examining lipid and phosphate modifications of RGS proteins. Series: Methods in Molecular Biology (Book 237) Hardcover: 250 pages Publisher: Humana Press; 2004 edition Language: English ISBN-10: 1588291375 ISBN-13: 978-1588291370 Product Dimensions: 6.1 x 0.6 x 9.2 inches Link Download http://nitroflare.com/view/223458BBA5DBB15https://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1