A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Series: Methods in Molecular Biology (Book 249) Paperback: 240 pages Publisher: Humana Press Language: English ISBN-10: 1617372692 ISBN-13: 978-1617372698 Product Dimensions: 6.1 x 0.6 x 9.2 inches Link Download http://nitroflare.com/view/BAD6E999A61220Chttps://drive.google.com/drive/folders/1yLBzZ1rSQoNjmWeJTZ3WGQHg04L1
